硼酸对非洲雏鸵鸟肺组织形态学的影响

doi:10.11843/j.issn.0366-6964.2015.12.022

Abstract

Abstract:

The aim of the present study was to detect the effects of boric acid on the morphology of African ostrich chick lung.Twenty-four healthy ostrich chicks were randomly divided into 6 groups：Group A(control group)，B，C，D，E，F，and supplemented with boric acid at the concentration of 0，40，80，160，320，and 640 mg•L^-1，respectively.The morphology changes of lung were observed by using light microscope and the HE staining technology，the collagen fiber changes were detected by MASSON staining，and TUNEL methods were used to label the apoptosis cells in ostrich lung.The results were as followings，compared with the control group，when the boric acid dose less than 80 mg•L^-1(group A，B，C)，the lung structure was well developed，cell morphology was intact，nucleus was stained darkly and the lung organizational structure was clearly，especially at 80 mg•L^-1.Adversely，when boric acid doses higher than 160 mg•L^-1(group D，E，F)，the high dose boric acid damaged the lung tissue structure，heterophilic granulocytes were increased，the interstitial was thickening with HE staining，especially at 640 mg•L^-1.MASSON staining showed that compared with the control group，collagen deposition was increased in high dose boric acid group(more then 320 mg•L^-1 boric acid).Compared with control group，the IOD of apoptosis cells was significantly decreased in the 40 mg•L^-1 boric acid group，whereas，the IOD of apoptosis cells was significantly evaluated in the 320 and 640 mg•L^-1 boric acid groups.Add 40 mg•L-1 boric acid can inhibit apoptosis in lung，more than 160 mg•L^-1 boric acid can promote inflammation，more than 320 mg•L^-1 boric acid can increase apoptosis and cause pulmonary fibrosis.

CLC Number:

S852.1

TANG Juan，XIAO Ke，ZHENG Xin-ting，ZHU Dai-yun，WANG Wei，WANG Jing，YANG Zhi，SUN Peng-peng，WANG Yan-hong，WANG Xiao-yi，PENG Ke-mei. Effect of Boric Acid on the Morphology of African Ostrich Chicks Lung[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(12): 2291-2298.

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Effect of Boric Acid on the Morphology of African Ostrich Chicks Lung

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